Detection of more than two different alleles per locus was observed for 34 accessions. Using 10 primer pairs of microsatellites, we were able to amplify 135 alleles for the 71 local apple accessions. These accessions were compared with 37 reference cultivars (24 traditional B&H and 13 international cultivars maintained at the ex situ collection Srebrenik) to determine synonyms, homonyms, and possible introgression of foreign genotypes into the local apple germplasm. Consequently, 71 local apple accessions (31 from Sarajevo and 40 from eastern Bosnia) were evaluated with a set of 10 simple sequence repeats (SSRs). There is a need for classifying and conserving local apple cultivars from two main regions in Bosnia and Herzegovina (B&H). | 7 Department of Horticulture, Oregon State University, 418 N.| 6 Norwegian Institute for Agricultural and Environmental Research, Bioforsk, Ullensvang, N-5781 Lofthus, Norway.| 5 Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, University of Zagreb, Svetosimunska 25, 10000 Zagreb, Croatia.| 4 Faculty of Agriculture and Food Sciences, University of Sarajevo, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and Herzegovina.| 3 Laboratory for Molecular Genetics of Natural Resources, Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, Kampus, 71 000, Sarajevo, Bosnia and Herzegovina.| 2 Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, University of Zagreb, Svetosimunska 25, 10000 Zagreb, Croatia.1 Faculty of Agriculture and Food Sciences, University of Sarajevo, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and Herzegovina.Authors: Fuad Gasi 1, Silvio Simon 2, Naris Pojskic 3, Mirsad Kurtovic 4, Ivan Pejic 5, Mekjell Meland 6, and Clive Kaiser 7 View More View Less
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